毛茛科植物黑种草(Nigella damascena)为生长于欧洲温带草原的常见草本植物。其种子芳香,与家黑种草(N. sativa)相同,在地中海以东国家被广泛用作面包和奶酪中的调味剂,民间用于调经。主要成分有生物碱、萜类和一些常见黄酮类化合物。据报道从黑种草种子极性溶剂提取物中分离出一些简单的酚性化合物。本次采用含有人雌激素受体的体外酵母雌激素筛选法(YES),检测了黑种草种子提取物及其酚性成分的雌激素活性。 The Ranunculaceae black plant grass (Nigella damascena) is a common herbaceous plant that grows on temperate grasslands in Europe. Its seed aroma, which is the same as that of N. sativa, is widely used as a flavoring agent in bread and cheese in the eastern Mediterranean countries, and is used for regulating menstruation. The main ingredients are alkaloids, terpenes and some common flavonoids. It was reported that some simple phenolic compounds were isolated from the polar solvent extracts of the black grass seeds. The in vitro yeast estrogen screening method (YES) containing the human estrogen receptor was used to detect the estrogenic activity of the black grass seed extract and its phenolic components.