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Photoaffinity cross-linking is a fast developing technology for biomolecular interactions,including receptor-ligand binding.The chemical mechanisms of the most commonly used photoactivatable probes and their respective photochemistry are summarized.This review focuses on the expanding utilities of this technology as a result of recent advances in the(i)identification of receptor contact sites,(ii)monitoring ligand-induced receptor conformational changes,(iii)identification of global binding surfaces,(iv)binding mode analysis using bifunctional photo-probes,(v)application of biosynthetic photo-probes,and(vi)examples of novel target discovery using this technology.Limitations and future potential of this approach are also discussed.
Photoaffinity cross-linking is a fast developing technology for biomolecular interactions, including receptor-ligand binding. The chemical mechanisms of the most commonly used photoactivatable probes and their own photochemistry are Summary. The review focuses on the expanding utilities of this technology as a result of recent advances in the (i) identification of receptor contact sites, (ii) monitoring ligand-induced receptor conformational changes, (iii) identification of global binding surfaces, (iv) binding mode analysis using bifunctional photo-probes, (v) application of biosynthetic photo-probes, and (vi) examples of novel target discovery using this technology. Limits and future potential of this approach are also discussed.