论文部分内容阅读
采用水平淀粉凝胶电泳技术研究中华稻蝗 (Oxyachinensis)四个种群的 12个基因座位 ,探讨其遗传分化。这四个种群分别采自内蒙古呼和浩特、山西代县、山西太原和陕西西安。Ck和Mdh 2在四个种群中均为单态 ,其余的基因座位至少在一个种群内有两个以上的等位基因 ;在Ldh和Mdh 1的等位基因频率呈现梯度分布趋势。多态基因座位百分率 (P)和平均每个基因座位的等位基因数目 (A)分别为 5 8 3% - 6 6 7%和 2 2 -2 8,平均杂合度为Ho=0 173- 0 2 4 0。除Gpi,Hk 2 ,Idh ,Ldh和Mdh 1在部分蝗虫种群符合Hardy Weinberg平衡外 ,其余绝大多数基因座位的基因型频率显著偏离Hardy Weinberg平衡。四个种群间FST平均值不存在显著差异 (FST=0 0 5 10 ,P >0 0 5 ) ,结合高的Nei’s遗传一致度 (I >0 97)可知 ,种群之间遗传分化不明显。我们认为人类的农业活动可能促进了种群间的基因交流 ,从而降低了分化程度
Horizontal starch gel electrophoresis was used to study the 12 loci in four populations of Oxyachinensis and to investigate their genetic differentiation. The four populations were collected from Inner Mongolia, Hohhot, Shanxi Dai County, Shanxi Taiyuan and Xi’an in Shaanxi. Ck and Mdh 2 were single in all four populations, and the rest of the loci had at least two alleles in at least one population. The allelic frequencies of Ldh and Mdh 1 showed a gradient distribution trend. The percentage of polymorphic loci (P) and the average number of alleles per locus (A) were 58.3% -6.67% and 2 2 -28, respectively. The average heterozygosity was Ho = 0 173- 0 2 4 0. Except for Gpi, Hk 2, Idh, Ldh and Mdh 1, most of the loci showed significant deviation from Hardy-Weinberg equilibrium except Hardy Weinberg equilibrium in some locust populations. There was no significant difference in FST between the four populations (FST = 0 0 5 10, P> 0 05). Combined with high Nei’s genetic identity (I> 0 97), the genetic differentiation among the populations was not obvious. We believe that human activities in agriculture may promote gene exchange among populations and thus reduce the degree of differentiation