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研究利用脂质体转染的方法,将小鼠B7基因导入C57BL小鼠大肠癌细胞株CMT93细胞(弱免疫原性),用800μg/ml的C418筛选得到阳性克隆,免疫组织化学检测发现B7分子得到充分表达,主要以细胞膜染色为主,也有部分细胞浆着色.将5×10~6B7~+CMT93细胞接种于C57BL小鼠背部皮下,以相同细胞数的野生型瘤细胞接种为阴性对照.结果发现实验组小鼠(n=4)第2周开始肿瘤的体积逐渐缩小,至第4周未肿瘤全部消失,而对照组肿瘤呈进行性生长(n=3),至第4周未肿瘤大小为(1.7654±0.4963)cm~2(长径×短径).实验组与对照组相比,有显著性差异(P<0.001).用B7~+CMT93瘤细胞致敏小鼠后再接种野生型的CMT93瘤细胞,小鼠全部不成瘤(n=4),未致敏小鼠除1只未成瘤外,余
To study the use of liposome transfection method, mouse B7 gene was introduced into C57BL mouse colorectal cancer cell line CMT93 cells (weak immunogenicity), positive clones were screened with 800 μg/ml of C418, and B7 molecule was detected by immunohistochemistry. It was fully expressed, mainly in cell membrane staining, and also in some cytoplasm staining. 5×10~6B7~+CMT93 cells were inoculated subcutaneously on the back of C57BL mice, and wild-type tumor cells with the same number of cells were vaccinated as negative controls. It was found that the tumor volume gradually decreased from the 2nd week of the experimental group (n=4) to the 4th week, while the control group showed progressive growth (n=3), and the tumor size did not reach the 4th week. It was (1.7654±0.4963)cm~2 (major diameter×short diameter). There was a significant difference between the experimental group and the control group (P<0.001). The mice were sensitized with B7~+CMT93 tumor cells and then inoculated wild. In the CMT93 tumor cells, all mice did not become tumors (n=4), except for one non-sensitized mouse that had not become tumorigenic.