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目前丝虫病的诊断方法仍以厚血膜法为主,此法操作简便,但在溶血和染色过程中微丝蚴容易脱落。我们用洗脱液微孔薄膜过滤法对此作了观察。 实验方法 1.对象及分组:检查班氏丝虫微丝蚴阳性者218例,阴性者63例,夜间9时至次晨1时取耳垂血60mm~3,涂成1m5×3cm的厚血膜(玻片均经严格处理),待干透后,将阳性者血片随机分为四组:(1)蒸馏水溶血组,观察66例。滴蒸馏水布满整个血膜,溶血10～20分钟。(2)吉氏液脱染组,观察53例。血
At present, the diagnosis of Filariasis is still based on the thick membrane method, which is easy to operate. However, microfilariae tend to fall off during hemolysis and staining. We used eluent microporous membrane filtration method for this observation. Experimental methods 1. Subjects and groups: check Bancroftian filarial microfilaria positive in 218 cases, 63 cases of negative, from 9:00 to 1:00 at the next morning to take earlobe blood 60mm ~ 3, painted 1m5 × 3cm thick membrane (Slides were strictly treated), to be dry, the positive blood film were randomly divided into four groups: (1) distilled water hemolysis group, observed 66 cases. Distilled water filled the entire blood film, hemolysis 10 to 20 minutes. (2) Kyrgyz liquid decolorization group, observed 53 cases. blood