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[目的]探究吡虫啉根部吸收后在小麦、棉花植株体内不同部位、不同时间的持留分布动态,为完善新烟碱类杀虫剂内吸测定方法提供实验依据。[方法]通过水培法处理小麦、棉花植株后,采用超高效液相色谱-串联质谱法(UPLC-MS/MS)对小麦、棉花植株不同部位、不同时间吡虫啉持留量进行测定。[结果]在相同的浓度下,测定时间范围内,小麦和棉花各部位吡虫啉持留量随着时间的延长而增加。在不同浓度下,随着时间的延长,植株地上各部分吡虫啉持留量均快速上升。随着药剂处理剂量的成倍增加,小麦植株体内吡虫啉的持留量似乎达到饱和,没有成比例增加。[结论]新烟碱类杀虫剂内吸生物测定方法是可行的,但需依据植株种类不同,明确试验药剂处理剂量、试验时间和植株供试部位。
[Objective] The research aimed to investigate the dynamics of the retention and distribution of imidacloprid root resorption in different parts of wheat and cotton at different time after inoculation, providing the experimental evidence for the improvement of the neonicotinoid insecticide. [Method] After the wheat and cotton plants were treated with hydroponics, the retention of imidacloprid in different parts of wheat and cotton plants was determined by UPLC-MS / MS. [Result] Under the same concentration, the retention of imidacloprid in wheat and cotton increased with the extension of time within the measuring time range. At different concentrations, with the extension of time, the imidacloprid retention of all parts of the plant increased rapidly. With dose doubling of the dose of treatment, the amount of imidacloprid remaining in the wheat plants appears to be saturated, not proportionally. [Conclusion] The neonicotinoid insecticide internal absorption bioassay method is feasible. However, it is necessary to test the dosage, test time and test site of the plant clearly according to the plant species.