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目的用HPLC分析胸腺法新的肽图。方法用TPCK-胰蛋白酶裂解和RP-HPLC法测定胸腺法新的肽图,用ESILC/MS技术分析肽段的精确相对分子量。结果根据肽段的色谱保留时间,相对分子质量归属肽图中肽段所在的色谱峰。结论所用方法精密度高、重复性好,可为胸腺法新的质量分析和控制提供依据。
Objective To analyze the new thymus peptide map by HPLC. Methods The new peptide map of thymus was determined by TPCK-trypsin cleavage and RP-HPLC, and the exact relative molecular weight of peptides was analyzed by ESILC / MS. Results According to the chromatographic retention time of the peptide, the relative molecular mass belongs to the peak of the peptide in the peptide map. Conclusion The method used has high precision, good repeatability and can provide the basis for the new quality analysis and control of thymus.