论文部分内容阅读
目的利用纤维素酶对苦参进行水解,以提高苦参有效成分的提取转移率、节省提取时间。方法在单因素试验基础上,以HPLC测得的苦参碱、氧化苦参碱的提取转移率为指标,采用星点试验设计,对加酶量(X1)、酶解pH(X2)和酶解时间(X3)3个因素(每个因素设5个水平)进行考察,优选苦参最佳酶解条件,并且以水提取方法作为对比,对酶解后的提取速度及次数进行考察。结果酶量为4 U/g、pH为5.6、温度为42℃时,纤维素酶对苦参的酶解效果最好,苦参碱、氧化苦参碱的提取转移率最高,在此条件下每次提取15 min,苦参碱、氧化苦参碱的提取转移率为87.89%,比水提取方法(不加酶、每次提取2 h)提高了7.03%,并节省了提取时间。结论纤维素酶的加入有利于苦参有效成分的溶出,节省提取时间,对工业生产有一定的意义。
Objective To hydrolyze Sophora flavescens by using cellulase in order to improve the extraction and transfer rate of the active ingredients of Sophora flavescens and save the extraction time. Methods Based on single factor experiments, the extraction and transfer rates of matrine and oxymatrine were determined by HPLC. The star point experiment design was used to determine the amount of enzyme (X1), enzyme pH (X2) and enzyme Solution time (X3) three factors (each set of five levels) were investigated, the optimal conditions Sophora hydrolysis conditions, and water extraction method as a comparison, the enzymatic extraction rate and the number of visits. Results Enzymatic amount of 4 U / g, pH 5.6, the temperature of 42 ℃, the best enzymatic hydrolysis of Sophora flavescens, matrine, oxymatrine extraction and transfer rate of the highest under this condition The extraction and transfer rate of matrine and oxymatrine was 87.89% for each extraction of 15 min, which was 7.03% higher than that of water extraction method (without adding enzyme for 2 h), and the extraction time was saved. Conclusion The addition of cellulase is beneficial to the dissolution of the active ingredients of Sophora flavescens, saving the extraction time and having some significance for industrial production.