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在pH4.7的HAc-NaAc缓冲溶液中,牛血红蛋白可催化H2O2氧化I-生成I2,I2与过量的I-生成的I3-与带正电荷的结晶紫(CV+)可形成缔合物微粒,导致体系的共振散射光强度增强。在659 nm处,H2O2在2.065×10-7～1.652×10-6mol/L范围内与共振散射光强度的增加值(ΔI)呈良好的线性关系,相关系数r为0.9989,检出限为2.676×10-9mol/L。据此,建立了检测痕量H2O2的共振散射光谱新方法,该方法已用于水样中H2O2含量的测定。
In pH4.7 HAc-NaAc buffer solution, bovine hemoglobin can catalyze H2O2 to oxidize I-to generate I2, I2 and excess I-generated I3- and positively charged crystal violet (CV +) to form association particles, Resulting in enhanced resonance scattering light intensity of the system. At 659 nm, H2O2 showed a good linear relationship with the increase of resonance light intensity (ΔI) in the range of 2.065 × 10-7 ~ 1.652 × 10-6 mol / L with a correlation coefficient of 0.9989 and a detection limit of 2.676 × 10-9mol / L. Based on this, a new resonance scattering method for the determination of trace H2O2 was established, which has been applied to the determination of H2O2 in water sample.