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目的:研究印记基因PEG10修饰的树突状细胞(DC)疫苗对肝癌细胞的杀伤效应,为肝癌的治疗提供新的策略。方法:将重组PEG10腺病毒rAd-PEG10感染HLA-A2阳性的人外周血来源的DC,制备PEG10基因修饰的DC疫苗,并在体外刺激HLA-A2阳性限制性的单个核细胞,酶联免疫斑点试验(Enzyme Linked Immunospot Assay,ELISPOT)和标准51Cr释放试验分别检测PEG10腺病毒感染的DC所诱导的特异性CTL活性,并检测对HLA-A2阳性的HepG2肝癌细胞的杀伤作用。结果:成功制备了PEG10基因修饰的树突状细胞(DC)疫苗,并在体外能有效诱导抗原特异性CTL效应,对HepG2肝癌细胞有明显的杀伤毒性。结论:PEG10基因修饰的树突状细胞能有效激发出特异性CTL应答,并对HepG2肝癌细胞有明显的杀伤毒性,为肝癌治疗提供了新思路。
Objective: To study the killing effect of imprinted gene PEG10 modified dendritic cell (DC) vaccine on hepatocellular carcinoma cells and to provide a new strategy for the treatment of liver cancer. Methods: Recombinant PEG10 adenovirus rAd-PEG10 was infected with HLA-A2 positive human peripheral blood derived DCs to prepare PEG10 gene-modified DC vaccine and stimulate HLA-A2 positive restrictive mononuclear cells in vitro. Enzyme Linked Immunospot Assay (ELISPOT) and standard 51Cr release assay were used to detect the specific CTL activity induced by DCs infected with PEG10 adenovirus and the killing effect on HepG2 hepatoma cells with HLA-A2 positive. Results: The dendritic cell vaccine modified by PEG10 gene was successfully prepared and effectively induced antigen-specific CTL effect in vitro, which was obviously cytotoxic to HepG2 hepatocarcinoma cells. Conclusion: Dendritic cells modified with PEG10 gene can effectively stimulate specific CTL responses and have obvious cytotoxicity to HepG2 hepatocarcinoma cells, providing new ideas for the treatment of hepatocellular carcinoma.