目的：研究苦参碱对小鼠脾细胞增殖及腹腔巨噬细胞释放白细胞介素１（ＩＬ１）及６（ＩＬ６）的影响．方法：［３Ｈ］ＴｄＲ参入法测定脾细胞增殖，胸腺细胞增殖法和Ｂ９细胞增殖ＭＴＴ法测定ＩＬ１和ＩＬ６活性．结果：苦参碱（１２５－５００ｍｇ·Ｌ－１）以剂量依赖方式显著抑制ＣｏｎＡ及脂多糖（ＬＰＳ）诱导的小鼠脾细胞增殖以及ＬＰＳ诱导的小鼠腹腔巨噬细胞释放ＩＬ１和ＩＬ６．结论：苦参碱抑制体外小鼠脾细胞增殖及巨噬细胞分泌ＩＬ１和ＩＬ６． Objective: To study the effects of matrine on the proliferation of mouse spleen cells and the release of interleukin1 (IL1) and 6 (IL6) by peritoneal macrophages. Methods: [3H] TdR incorporation method was used to determine the activity of IL1 and IL6 in spleen cell proliferation, thymocyte proliferation and B9 cell proliferation MTT assay. Results: Matrine (125-500mg · L-1) significantly inhibited the proliferation of mouse splenocytes induced by ConA and lipopolysaccharide (LPS) in a dose-dependent manner and the release of IL1 and IL by LPS-induced mouse peritoneal macrophages  6. Conclusion: Matrine can inhibit the proliferation of murine splenocytes in vitro and the secretion of IL1 and IL6 by macrophages.