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目的:以HepG2.2.15细胞株为实验模型,研究乙肝扶正排毒胶囊对乙肝病毒e抗原(HBeAg)、表面抗原(HB sAg)、脱氧核糖核酸(HBV-DNA)的抑制作用及机理探讨和对NF-kb活化效应研究。方法:通过细胞毒性实验以确定对细胞无毒性作用的最高药物浓度,加药物和含药血清到细胞培养液中,分别采取第4天、第8天的细胞培养上清液,用ELISA法测定其HBsAg、HBeAg、HBV-DNA含量,用免疫组化检测方法比较各组细胞模型之间NF-kb活化阳性细胞率。结果:乙肝扶正排毒胶囊最大无毒浓度TD0<2.81mg/ml,乙肝扶正排毒胶囊以及其含药血清有明显抑制HBsAg、HBeAg、HBV-DNA的作用,各浓度间存在量效关系,且NF-kb活化阳性细胞率也大于其他对照各组。结论:乙肝扶正排毒胶囊对HepG2.2.15细胞株HBV标志物有显著的抑制作用;其抑制作用机理和效果与NF-kb活化程度相关。
Objective: To study the inhibitory effect and mechanism of hepatitis B virus e antigen (HBeAg), surface antigen (HB sAg), and deoxyribonucleic acid (HBV-DNA) on HepG2.2.15 cell line as an experimental model and to NF Study on the activation effect of -kb. Methods: The highest drug concentration without cytotoxic effect on cells was determined by cytotoxicity test. Drug and drug-containing serum were added to cell culture medium. Cell culture supernatants were taken on the 4th and 8th days, respectively, and determined by ELISA. The HBsAg, HBeAg, and HBV-DNA contents were compared using immunohistochemistry to compare the rate of NF-kb activation positive cells among cell models in each group. RESULTS: The maximum non-toxic concentration of hepatitis B Fuzheng Paidu capsule was TD0<2.81mg/ml. The hepatitis B Fuzheng detoxification capsule and its drug-containing serum significantly inhibited the effects of HBsAg, HBeAg and HBV-DNA. There were dose-effect relationships among the concentrations, and NF- The rate of kb activated positive cells was also greater than that of other control groups. Conclusion: Hepatitis B Paiduodu capsule has a significant inhibitory effect on HBV markers in HepG2.2.15 cell line; its inhibitory mechanism and effect are related to the degree of NF-kb activation.