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用5.2mg/mL(LC50)的印楝种子提取物对荔枝蝽1龄若虫进行急性毒性处理,24h死亡率为51.8%。通过等位酶分析检测了死亡与存活试虫两种酶(PGI和MDH),两个基因座(Pgi和Mdh)上各基因型及等位基因与印楝种子提取物毒性之间的关系,进行致死性差异比较研究。结果表明,印楝种子提取物对具有不同基因型及等位基因个体的致死性存在差异。在Pgi基因座上,Pgi-bb基因型死亡率最高,为84%,Pgi-aa和Pgi-cc基因型死亡率较低,分别为0和7%,且与死亡率最高的Pgi-bb基因型存在显著差异(P<0.05)。在基因座Mdh上,Mdh-aa基因型个体死亡率最高(93%),而具有Mdh-cc基因型的个体全部存活了下来,另外3个基因型Mdh-ab、Mdh-bb与Mdh-bc死亡率居中,都与Mdh-aa、Mdh-cc基因型死亡率之间存在显著差异。在等位基因上,Pgi-a和Mdh-c个体的死亡率都最低,与各自其他两个等位基因的死亡率之间存在显著差异。结果说明不同基因型个体对印楝提取物具有不同的反应,印楝种子提取物对荔枝蝽等位酶基因型及等位基因存在选择性致死作用。这种荔枝蝽对印楝种子提取物的敏感性与其等位酶基因型及等位基因之间显明的相关关系提示我们,可将荔枝蝽种群中对印楝种子提取物敏感性低的基因型及等位基因作为遗传标记去监测荔枝蝽对印楝种子提取物的抗性状况。
The first instar nymphs of litchi stinkbug were treated with 5.2mg / mL (LC50) of neem seed extract for 24 hours and the mortality rate was 51.8%. The relationship between the two genotypes (PGI and MDH), the genotypes and alleles at two loci (Pgi and Mdh) and the virulence of neem seed extract was tested by allelic enzyme analysis. To compare the fatal differences. The results showed that neem seed extract of different genotypes and alleles of individual lethality there are differences. The Pgi-bb genotype had the highest mortality at the Pgi locus, 84%, and the Pgi-aa and Pgi-cc genotypes had lower mortality rates of 0 and 7%, respectively, and was associated with the highest-mortality Pgi-bb gene There was significant difference (P <0.05). Mdh-aa genotype had the highest mortality (93%) at locus Mdh, while all individuals with Mdh-cc genotype survived. The other three genotypes Mdh-ab, Mdh-bb and Mdh-bc The median mortality was significantly different from that of Mdh-aa and Mdh-cc genotypes. On the allele, Pgi-a and Mdh-c individuals had the lowest mortality rates, which were significantly different from those of the other two alleles. The results showed that individuals of different genotypes had different responses to neem extract. Neem seed extract had selective lethal effect on the isozymes and alleles of litchi stink bug. The significant correlation between the susceptibility of Neisseria gonorrhoeae to the neem seed extract and its allelic genotypes and alleles suggests that we can isolate the genotypes with low sensitivity to neem seed extract And alleles as genetic markers to monitor litchi stinkbug neem seed extract resistance status.