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探讨煤烟导致细胞体系氧化应激损伤的机制。 [方法 ]以N 乙酰半胱氨酸 (NAC)和甘露醇等不同作用位点的抗氧化剂作为实验干预手段 ,采用MTT比色法和溴乙锭荧光法观察煤烟所致细胞毒性和肺细胞DNA交联形成作用。 [结果 ]N 乙酰半胱氨酸 (NAC)可以有效地降低煤烟所致的细胞毒性和细胞DNA交联形成作用 ;而甘露醇却没有观察到上述作用。 [结论 ]煤烟在细胞内经代谢活化后产生的某些代谢产物可以降低体内谷胱甘肽含量 ,引起细胞DNA的氧化应激损伤。
To explore the mechanism of soot-induced oxidative stress in the cellular system. [Method] With antioxidants such as N-acetylcysteine (NAC) and mannitol as experimental interventions, MTT colorimetry and ethidium bromide fluorescence were used to observe the cytotoxicity induced by soot and the changes of lung cells DNA cross-linking formation. [Results] N-acetylcysteine (NAC) could effectively reduce the cytotoxicity and cellular DNA cross-linking effect caused by soot; however, no effect was observed with mannitol. [Conclusion] Some metabolites produced by the metabolism of soot in the cells can reduce the content of glutathione in the body and cause the oxidative stress damage of DNA.