论文部分内容阅读
目的 探讨CYP2D6基因和UGT1A6基因多态性与慢性苯中毒易感性的关系。方法 采用病例-对照研究, 选择152名苯中毒工人为病例组, 152名接触苯而无中毒表现的工人为对照组。采用PCR RFLP技术检测CYP2D6第9外显子g.4268位点和UGT1A6第1外显子t.181位点的多态性。结果 携带CYP2D6 g.4268 C/C基因型的个体较携带有CYP2D6 g.4268 G/G或G/C基因型的个体发生苯中毒的危险性高1 72倍(95%CI: 1 08~2 78, P=0 03); 在不吸烟的人群中, 携带CYP2D6 g.4268 C/C基因型的个体比携带G/G或G/C基因型的个体发生苯中毒的风险性高1 75 倍(95%CI:1 03~2 94,P=0 04); 在不饮酒的人群中, 携带CYP2D6 g.4268 C/C基因型个体比携带CYP2D6 g.4268 G/G和G/C基因型的个体发生苯中毒的危险性高1 82倍(95% CI:1 09~3 03,P=0 02)。UGT1A6 t.181 的各基因型在病例组和对照组的分布频率差异无显著性(P>0 05)。结论 携带CYP2D6 g.4268 C/C基因型的个体对苯中毒可能易感。
Objective To investigate the relationship between CYP2D6 gene and UGT1A6 gene polymorphism and the susceptibility to chronic benzene poisoning. Methods A case-control study was conducted in which 152 workers with benzene poisoning were selected as the case group and 152 workers exposed to benzene without toxicity were selected as the control group. PCR RFLP was used to detect the polymorphism of g.4268 in exon 9 of CYP2D6 and t.181 in exon 1 of UGT1A6. Results Individuals carrying the CYP2D6 g.4268 C / C genotype had a 72-fold higher risk of benzene poisoning than those carrying the CYP2D6 g.4268 G / G or G / C genotype (95% CI: 1 08-2 78, P = 0 03). Among non-smokers, individuals with CYP2D6 g.4268 C / C genotype had a 1.75-fold higher risk of benzene poisoning than those with G / G or G / C genotypes (95% CI: 103-294, P = 044). In non-alcoholic subjects, individuals carrying the CYP2D6 g.4268 C / C genotype had higher CYP2D6 g.4268 G / G and G / C genotype Of individuals had a 181-fold higher risk of benzene poisoning (95% CI: 109-03, P = 0.02). There was no significant difference in the distribution frequencies of UGT1A6 t.181 between the case group and the control group (P> 0.05). Conclusion Individuals with CYP2D6 g.4268 C / C genotype may be susceptible to benzene poisoning.