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为进一步研究草莓(Fragaria×ananassa)芽休眠诱导过程中温度和光周期响应的互作关系,以草莓Albion盆栽匍匐茎苗为试材,分别实施18℃/8h、15℃/16h、15℃/8h和12℃/16h处理,以26℃/16h为对照,光照强度是200μmol/(m2·s),调查叶生长和相关基因表达量。结果低温、短日均使相邻两片叶抽生的时间间隔明显延长,抑制草莓叶生长。15℃/8h和12℃/16h温光组合可以有效诱导草莓植株假休眠。15℃临界温度下FaPHYA和FaPHYB表达受短日照诱导,通过FaPIF4调控IAA响应,较高温度下植株对日长的响应延迟。而12℃条件下草莓植株休眠诱导与光周期反应不相关。低温与短日均为草莓芽休眠诱导的环境因子,温度较光周期更重要。
In order to further study the interaction between temperature and photoperiod response during the dormancy induction of strawberry (Fragaria × ananassa), the stalks of Albion were used as test materials, and the effects of 18 ℃ / 8h, 15 ℃ / 16h, 15 ℃ / 8h and 12 ℃ / 16h treatment, 26 ℃ / 16h as control, light intensity was 200μmol / (m2 · s), leaf growth and related gene expression levels were investigated. Results Low temperature and short day both prolonged the time interval between leaf pumping of two adjacent leaves and inhibited the growth of strawberry leaves. 15 ℃ / 8h and 12 ℃ / 16h temperature and light combinations can effectively induce false dormancy in strawberry plants. The expression of FaPHYA and FaPHYB was induced by short day at the critical temperature of 15 ℃, and the IAA response was regulated by FaPIF4. The response of plants to higher temperature was delayed at higher temperature. However, the dormancy induction of strawberry plants at 12 ℃ was not related to the photoperiodic response. Low temperature and short day are the environmental factors induced by dormancy of strawberry buds, and the temperature is more important than the photoperiod.