论文部分内容阅读
目的:探讨京尼平苷对UVA损伤ESF-1细胞的保护作用及其机理。方法:用20 J/cm2的UVA照射ESF-1细胞建立损伤模型,以不同浓度京尼平苷处理损伤细胞,用试剂盒分别检测细胞中SOD、GSH-Px、CAT活性和MDA含量,RT-PCR法检测细胞中ERK1/2、MMP-1及TIMP-1 mRNA的表达量,ELISA法检测细胞上清液中MMP-1、TIMP-1的含量。结果:UVA照射后,ESF-1细胞上清液中SOD活性、GSH-Px活性、CAT活性、TIMP-1 mRNA的表达量、TIMP-1含量显著降低,MDA含量、ERK1/2、MMP-1 mRNA的表达量、MMP-1含量显著升高(P<0.01);5×10-5mol/L和5×10-6mol/L京尼平苷能显著改善ESF-1细胞的损伤(P<0.05或P<0.01)。结论:京尼平苷能减轻UVA对ESF-1细胞的损伤,其机制可能与抑制氧化损伤,调控ERK信号通路,调节MMP-1和TIMP-1的表达有关。
Objective: To investigate the protective effect and mechanism of geniposide on ESA-1 cells damaged by UVA. Methods: The injury model was established by ESA-1 cells exposed to UVA at a dose of 20 J / cm2. The injured cells were treated with different concentrations of geniposide. The activities of SOD, GSH-Px, CAT, The expression of ERK1 / 2, MMP-1 and TIMP-1 mRNA in the cells was detected by PCR and the levels of MMP-1 and TIMP-1 in the supernatant of the cells were measured by ELISA. Results: After UVA irradiation, the activity of SOD, the activity of GSH-Px, the activity of CAT, the expression of TIMP-1 mRNA, the content of TIMP-1 in ESF-1 cell supernatant were significantly decreased, the content of MDA, ERK1 / 2 and MMP- (P <0.01); 5 × 10-5mol / L and 5 × 10-6mol / L of geniposide can significantly improve the injury of ESF-1 cells (P <0.05 Or P <0.01). CONCLUSION: Geniposide can reduce the damage of ESA-1 cells induced by UVA, which may be related to the inhibition of oxidative damage, the regulation of ERK signaling pathway and the regulation of MMP-1 and TIMP-1 expression.