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目的 探讨ＰＣＮＡ 和Ａｇ ＮＯＲ 在胃肠道平滑肌肿瘤组织中的表达及其与肿瘤良恶性的关系。方法 采用免疫组化ＳＰ 法和Ｈｏｗ ｅｌｌ 银染一步法检测６０ 例病理形态典型的胃肠道平滑肌肿瘤的ＰＣＮＡ 和Ａｇ ＮＯＲ 。结果 平滑肌肉瘤ＰＣＮＡ 指数明显高于平滑肌瘤，两者间有非常显著性差异（ Ｐ ＜ ０ ．０１） 。平滑肌肉瘤Ａｇ ＮＯＲ 平均含量（ 每个细胞内的平均颗粒数） 和颗粒直径（μｍ ） 分别为（８ ．２５ ±０ ．９２） μｍ 和（２ ．９０ ±０ ．２３） μｍ ，平滑肌瘤则分别为（２ ．８６ ±０ ．２９） μｍ 和（２ ．１２ ±０ ．１４） μｍ ，两者有非常显著性差异（ Ｐ＜ ０ ．０１） 。结论 ＰＣＮＡ 指数和Ａｇ ＮＯＲ 颗粒数及颗粒直径可作为鉴别胃肠道平滑肌肿瘤良恶性的客观指标之一。
Objective To investigate the expression of PCNA and Ag NOR in gastrointestinal smooth muscle tumors and its relationship with benign and malignant tumors. Methods Immunohistochemical SP method and Howell silver staining were used to detect PCNA and Ag NOR in 60 cases of pathologically typical gastrointestinal smooth muscle tumors. Results The PCNA index of leiomyosarcoma was significantly higher than that of leiomyoma. There was a significant difference between the two (P < 0.01). The mean Ag NOR content (average number of particles per cell) and particle diameter (μm) of leiomyosarcoma was (8.25 ± 0.92) μm and (2.90 ± 0.23) μm, respectively. They were (2.86 ±0.29) μm and (2.12 ±0.14) μm, respectively. There was a very significant difference between the two (P < 0. 01). Conclusions PCNA index, Ag NOR particle number and particle diameter can be used as one of the objective indicators to distinguish benign and malignant gastrointestinal smooth muscle tumors.