A well designed continuous annular chromatograph(CAC) column was set to study the enrichment of Boron isotopes. The column is 820 mm high and consists of a rotational annulus with an outer diameter of 246 mm and a width of 15 mm. The weak basic resin(Diaion WA21J), the boric acid and water were used as the absorbent, the feed and the eluent respectively. The effects of CAC rotating speed, concentration of boric acid and flow ratio of eluent to feed on the enrichment of 10 B were investigated. The concentration of boron isotope was determined by a Thermal Elemental X7 ICP-MS(Thermo Electron Co., USA). It is shown from the experimental results that 10 B and 11 B can be separated effectively by this CAC column. By fitting the experimental elution profiles based on reliable mathematic models and software the mass transfer coefficient k and adsorption equilibrium constant K values of 10 B and 11 B in CAC column were estimated. It is clear from this study that the CAC has practical continuous operational capabilities in comparison with fixed batch chromatography and is an effective technology for separating boron isotopes. The well is a continuous circular chromatograph (CAC) column was set to study the enrichment of Boron isotopes. The column is 820 mm high and consists of a rotational annulus with an outer diameter of 246 mm and a width of 15 mm. The weak basic resin (Diaion WA21J), the boric acid and water were used as the absorbent, the feed and the eluent respectively. The effects of CAC rotating speed, concentration of boric acid and flow ratio of eluent to feed on the enrichment of 10 B were investigated. The concentration of boron isotope was determined by a Thermal Elemental X7 ICP-MS (Thermo Electron Co., USA). It is shown from the experimental results that 10 B and 11 B can be separated effectively by this CAC column. By fitting the experimental elution profiles based on reliable mathematic models and software the mass transfer coefficient k and adsorption equilibrium constant K values ​​of 10 B and 11 B in CAC column were estimated. It is clear from this study that the CAC has practical continuou s operational capabilities in comparison with fixed batch chromatography and is an effective technology for separating boron isotopes.