目的:研究药对丹参-红花对脑缺血再灌注损伤大鼠血清中脑源性神经营养因子(BDNF)、胶质细胞源性神经营养因子(GDNF)表达的影响,并探讨该药对对此模型大鼠脑保护的作用机制。方法:采用改良Zea-Longa法制备大鼠局灶性大脑中动脉阻塞再灌注(MCAO/R)模型,将造模成功大鼠随机分为假手术组,模型对照组,尼莫地平组,丹参组,红花组,丹参-红花组(6.48g/kg、3.24g/kg、1.62g/kg)。然后对大鼠进行神经功能评分,观察大鼠脑组织病理形态学变化,并测定血清中BDNF、GDNF含量的变化情况。结果:与其他各组大鼠比较,丹参-红花6.48g/kg、3.24g/kg剂量组大鼠神经功能评分结果显著降低,脑组织结构改善明显,尼氏小体数量显著增高,血清中BDNF、GDNF含量表达明显升高。结论:药对丹参-红花对脑缺血再灌注损伤大鼠具有保护作用,其中6.48g/kg、3.24g/kg剂量组疗效最佳。 Objective: To study the effect of Salvia Miltiorrhiza on the expression of serum brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) in rats with cerebral ischemia-reperfusion injury, The mechanism of brain protection in this model rats. Methods: The focal middle cerebral artery occlusion and reperfusion (MCAO / R) model was prepared by the modified Zea-Longa method. The rats were randomly divided into sham-operation group, model control group, nimodipine group, Group, safflower group, Salvia - safflower group (6.48g / kg, 3.24g / kg, 1.62g / kg). The rats were then scored for neurological function to observe the pathological changes in the brain tissue of rats, and the changes of serum BDNF and GDNF levels were measured. Results: Compared with other groups, the scores of neurological function in the groups of Danshen-safflower 6.48g / kg and 3.24g / kg were significantly decreased, the structure of brain tissue was obviously improved and the number of Nissl bodies was significantly increased, BDNF, GDNF expression increased significantly. Conclusion: The medicine has the protective effect on Salvia miltiorrhiza - safflower on cerebral ischemia-reperfusion injury in rats, of which 6.48g / kg, 3.24g / kg dose group the best effect.