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测定循环中免疫复合物(IC)的含量有利于诊断免疫复合物病。迄今检测IC的方法有聚乙二醇(PEG)沉淀法、Clq凝胶扩散法,Raji细胞放射免疫法、~(125)I—Fab、mRF凝胶扩散法等,本文采用3.75％PEG沉淀法检测了慢性活动性肝炎等疾病,现把结果报道如下。 一、材料与方法 (一)检测方法:取受检血清0.2ml,加0.1M pH8.4硼酸缓冲液0.4ml使成1:3稀释。对照管加2ml同一硼酸缓冲液和0.22ml稀释血清。测定管中加2ml 4.166％PEG(上海合成洗涤二厂产品、批号79—10—20)溶液和0.22ml稀释血清,此时PEG浓度为3.75％。混和,置室温60分钟后用
Determination of circulating immune complexes (IC) content is conducive to the diagnosis of immune complex disease. So far, IC methods such as PEG precipitation, Clq gel diffusion, Raji cell radioimmunoassay, ~ (125) I-Fab, mRF gel diffusion and so on are used in this paper. Detection of chronic active hepatitis and other diseases, now the results reported below. First, the material and methods (A) detection method: Take seized serum 0.2ml, add 0.1M pH8.4 borate buffer 0.4ml into 1: 3 dilution. Control tube plus 2 ml of the same borate buffer and 0.22 ml of diluted serum. Assay tube 2ml 4.166% PEG (Shanghai Synthetic Wash Plant product, lot number 79-10-20) solution and 0.22ml diluted serum, PEG concentration at this time was 3.75%. Mix, set at room temperature after 60 minutes