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目的：研究西洋参多糖（ＰＰＱ）中均一组分ＰＰＱ５２的分离、糖组成、相对分子量及生物活性。方法：ＳｅｐｈａｄｅｘＤＥＡＥＡ５０和ＳｅｐｈａｄｅｘＧ１００分离，气相色谱法测定糖组成，基底辅助激光解吸／电离质谱测定相对分子量。用小鼠脾淋巴细胞进行淋巴细胞转化实验和白介素２促诱生实验。结果：ＰＰＱ５２由Ｄ阿拉伯糖和Ｄ半乳糖以１∶１．１摩尔比组成，含糖醛酸，相对分子量２１０００Ｄ，具有刺激淋巴细胞转化及白介素２促诱生功能。结论：由西洋参中可得到具有免疫刺激活性的含糖醛酸多糖
Objective: To study the separation, saccharide composition, relative molecular weight and biological activity of PPQ5, a homogenous component of American ginseng polysaccharide (PPQ). METHODS: Sephadex DEAEA 50 was separated from Sephadex G100. The saccharide composition was determined by gas chromatography and the relative molecular weight was determined by basal-assisted laser desorption/ionization mass spectrometry. Using mouse spleen lymphocytes for lymphocyte transformation experiments and interleukin 2 pro-induction experiments. RESULTS: PPQ5-2 was composed of D-arabinose and D-galactose in a ratio of 1:1.1, containing uronic acid, relative molecular mass of 21000D, and had the function of stimulating lymphocyte transformation and interleukin-2 provocation. Conclusion: The uronic polysaccharides with immunostimulatory activity can be obtained from American ginseng