论文部分内容阅读
以株-1S、SV1S、SV14S为研究材料,分析这3种材料的胚乳α-淀粉酶活性、第二叶鞘及节间长度对赤霉素(GA3)反应,结果表明来源于株-1S的SV1S、SV14S突变体的矮化变异与赤霉素信号传导途径无明显关系.此外,通过3种材料的基因组DNA和线粒体DNA的RAPD分析,发现矮秆突变体株系的基因组DNA和线粒体DNA都存在一定的变化,从而在DNA分子水平上进一步证实了SV1S和SV14S突变体的遗传突变.其突变的生理机制与赤霉素信号传导途径无明显关系,很可能与赤霉素生物合成途径有关.
Strain-S1, SV1S and SV14S were used as materials to analyze the endosperm α-amylase activity and the second leaf sheath and internode length to gibberellin (GA3). The results showed that SV1S , There was no significant relationship between the dwarf variation of SV14S mutant and gibberellin signaling pathway.In addition, genomic DNA and mitochondrial DNA of dwarf mutant lines were found by RAPD analysis of genomic DNA and mitochondrial DNA of three kinds of materials The results showed that the mutation of SV1S and SV14S had no significant relationship with GA3 signal transduction pathways, which may be related to GA3 biosynthesis pathway.