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全胚胎体外培养技术是将孕第９．５天的大鼠胚胎从子宫移至体外培养，培养期间在培养基中加入不同的化合物，由于排除了母体和胎盘因素，可以观察到这些化合物对胚胎生长发育的直接影响。应用该技术研究了青霉素、敌枯双、放线菌素Ｄ和高浓度葡萄糖四种化合物对胚胎生长发育的直接影响，结果发现在体外培养时也可导致胚胎死亡、畸形和胚胎生长发育迟缓，所导致的畸形与在整体动物实验结果相同。同时发现化合物在导致胚胎死亡、畸形和生长发育迟缓方面具有明显的剂量－效应关系。通过比较这种化合物导致胚胎死亡、畸形和发育迟缓的剂量－效应曲线的关系，推测其导致胚胎畸形的可能性以及畸形胚胎存活的可能性，从而推测化合物在实际中真正致畸的可能性。
Whole-embryo in vitro culture technique involves the transfer of rat embryos from the uterus on day 9.5 of gestation to in vitro culture with the addition of different compounds to the culture medium. The exclusion of maternal and placental factors has led to the observation of these compounds on embryos The direct impact of growth and development. The direct effect of four compounds including penicillin, dizocarb, dactinomycin and high concentration of glucose on the growth and development of embryos was studied. The results showed that embryo death, deformity and embryo growth retardation could be caused by in vitro culture. The resulting deformities are the same as the overall animal experiment results. At the same time, compounds were found to have a significant dose-effect relationship in causing embryonic death, malformations and growth retardation. The potential for teratogenicity and the possibility of survival of deformed embryos were speculated by comparing the dose-response curves of the compounds that led to embryonic death, deformity, and stunting to speculate about the potential for teratogenicity of the compound in practice.