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[目的]了解河南省艾滋病病毒-1型(human immunodeficiency virus-1,HIV-1)耐药毒株传播情况。[方法]通过横断面调查,采集年龄在16～60岁之间于2009年1～8月首次确诊的HIV-1型感染者血浆样本,用逆转录聚合酶链式反应(Reverse Transcription Polymerase Chain Reaction,RT-PCR)方法扩增HIV-1pol区基因,进行基因型耐药分析。[结果]141例采集样本中,扩增并测序成功104例,在逆转录酶(Reverse transcription,RT)区检测出8例针对逆转录酶抑制剂(Reverse Transcription Inhibitors,RTIs)的13个耐药突变位点,蛋白酶(protease,PR)区未检测出耐药突变位点。7.7%(8/104)的样本检测出耐药毒株且50%(4/8)的耐药毒株源自固定性伴性传播。[结论]河南省HIV-1耐药株的传播与以往相比处于明显上升势态,应加强治疗前的耐药性监测,适当调整用药方案和对单阳配偶家庭或固定性伴采取相应的干预措施。
[Objective] To understand the transmission of drug-resistant HIV-1 strains in Henan Province. [Methods] The plasma samples of HIV-1 infected persons aged 16-60 years old who were diagnosed for the first time from January to August in 2009 were collected by cross-sectional investigation. The reverse transcription polymerase chain reaction , RT-PCR) was used to amplify the HIV-1pol gene and analyze its genotypes. [Results] Of the 141 samples collected, 104 were successfully amplified and sequenced, and 8 of them were detected in Reverse transcription (RT) region against 13 Reverse Transcription Inhibitors (RTIs) Mutation sites, protease (protease, PR) region did not detect drug-resistant mutation sites. In 7.7% (8/104) of the samples, drug-resistant strains were detected and 50% (4/8) of the drug-resistant strains were derived from fixed-sex sexual transmission. [Conclusion] The transmission of HIV-1-resistant strains in Henan Province was significantly higher than that in the past. The monitoring of drug resistance before treatment should be strengthened, the medication regimen should be appropriately adjusted and the corresponding intervention should be taken on single-spouse family or fixed partners Measures.