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目的:观察龟鹿二仙胶、补肾健脾汤等不同补肾方对去卵巢骨质疏松模型大鼠骨质疏松的影响及干预作用。方法:取3月龄SPF级雌性SD大鼠70只,随机分为假手术组、模型组、龟鹿二仙胶组、二仙汤组、补肾健脾汤组、雌二醇组和仙灵骨葆组,每组10只。对假手术组大鼠行假手术,余6组行双侧卵巢摘除术,12周后测定各组大鼠血清中Ⅰ型胶原羧基端肽(β-Crosslaps)、总Ⅰ型胶原氨基端延长肽(P1NP),以双能X线骨密度测定仪测定大鼠的骨密度(BMD)。结果:与雌二醇组比较,龟鹿二仙胶治疗组、补肾健脾组、仙灵骨葆组BMD显著增高(P<0.05);二仙汤组、仙灵骨葆组β-Crosslaps含量显著降低(P<0.05),补肾健脾汤组P1NP含量显著增高(P<0.05);与二仙汤组比较,龟鹿二仙胶组、补肾健脾汤组BMD显著增高(P<0.05);补肾健脾汤组β-Crosslaps及P1NP含量均显著增高(P<0.05),龟鹿二仙胶组P1NP含量显著增高(P<0.05)。结论:龟鹿二仙胶、补肾健脾汤补肾中药可以促进骨形成,减缓骨吸收,能有效防止去卵巢模型大鼠骨质疏松的形成。
OBJECTIVE: To observe the effects of different tonifying kidney invigorating herbs such as Guilu Erxian, Bushen Jianpi Decoction on osteoporosis in ovariectomized osteoporosis rats and its intervention. Methods: Seventy SPF female SD rats of 3 months old were randomly divided into sham operation group, model group, Guilu Eryngsian Group, Erxian Decoction group, Bushen Jianpi Decoction group, estradiol group and Xianling Gu Bao group, each group of 10. The rats in sham operation group were sham operated and the other 6 groups were ovariectomized. After 12 weeks, the serum levels of type Ⅰ collagen carboxy-terminal (β-Crosslaps) and type Ⅰ collagen (P1NP). The bone mineral density (BMD) of rats was measured by dual energy X-ray absorptiometry. Results: Compared with estradiol group, the BMD of Guilu Erxian gum group, Bushen Jianpi group and Xianlinggubao group were significantly increased (P <0.05); β-Crosslaps content of Erxian decoction group and Xianling Gubao group (P <0.05). The content of P1NP in Bushen Jianpi Decoction group was significantly higher than that in Erxian Decoction group (P <0.05) (P <0.05). The content of P1NP in Busujianxin group was significantly higher than that in Busujianpi Decoction (P <0.05). Conclusion: Guilu Erxian gum and Bushen Jianpi Tang Bushen Chinese herbs can promote bone formation, reduce bone resorption and effectively prevent the formation of osteoporosis in ovariectomized rats.