论文部分内容阅读
为了进一步了解黑龙江省部分牛场致犊牛腹泻大肠杆菌毒力因子的流行情况,试验采用常规分离和PCR检测方法对2014—2015年黑龙江省部分牛场采集的72份犊牛病料进行检测,鉴定得到63株大肠杆菌,并应用PCR技术对CS31A、K88、K99、F17、F41、irp2、eae A、sta、lt、bfp A、EAST1等毒力因子进行检测。结果表明:有46株大肠杆菌含有毒力因子,通常含1~2种毒力因子,其中有18株携带(占39.13%)CS31A基因,说明CS31A基因为优势基因;通过毒力因子对大肠杆菌进行分型,共鉴定出产肠毒素性大肠杆菌(ETEC)35株(占76.09%),为犊牛腹泻的主要致病型。
In order to further understand the prevalence of E. coli virulence factors caused by diarrhea of calves in some cattle farms in Heilongjiang Province, 72 calf disease materials collected from some cattle farms in Heilongjiang Province during 2014-2015 were tested by routine isolation and PCR detection methods. 63 strains of Escherichia coli were identified and the virulence factors of CS31A, K88, K99, F17, F41, irp2, eae A, sta, lt, bfp A and EAST1 were detected by PCR. The results showed that 46 Escherichia coli strains contained virulence factors, usually containing 1-2 kinds of virulence factors, of which 18 strains (39.13%) carried CS31A gene, indicating that CS31A gene was the predominant gene. By virulence factor, 35 strains of enterotoxigenic Escherichia coli (ETEC) were identified (76.09%), which were the main pathogenic types of calf diarrhea.