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在广东发现了可能被番茄斑萎病毒属(Tospovirus)病毒侵染的西瓜,采用ELISA和RT-PCR法对该西瓜病样进行了检测,西瓜病叶粗汁液不与番茄斑萎病毒(Tomato spotted wilt virus,TSWV)和西瓜银斑驳病毒(Watermelon silver mottle virus,WSMoV)的血清发生反应;利用引物J13/UHP通过RT-PCR可以扩增出约1400 bp的基因片段,该片段包括一个840 bp的核衣壳蛋白ORF,其推导的氨基酸序列与已报道的Melon yellow spot virus(MYSV)NP基因氨基酸序列的同源率都为99%,进化树分析表明侵染广东西瓜的病毒(命名为MYSV-GZ)属于Tospovirus的MYSV血清组。
The watermelon that may have been infected by the virus of Tospovirus was found in Guangdong. The watermelon was detected by ELISA and RT-PCR. The watery juice of watermelon was not associated with Tomato spotted virus wilt virus (TSWV) and watermelon silver mottle virus (WSMoV) were detected by RT-PCR. A 1400 bp gene fragment was amplified by RT-PCR using primer J13 / UHP. The fragment contains a 840 bp The deduced amino acid sequence of the nucleocapsid protein ORF was 99% homologous to the reported amino acid sequence of the Melon yellow spot virus (MYSV) NP gene. Phylogenetic tree analysis indicated that the virus (named as MYSV- GZ) belongs to the MYSV serogroup of Tospovirus.