以细菌人工染色体pECBAC1为载体 ,构建了野生一粒小麦 (TriticumboeoticumBoiss)的基因组BAC文库。该文库共包含约 17万个克隆 ,平均插入片段长度为 10 4kb ,按野生一粒小麦基因组为 5 6 0 0Mb计算 ,文库覆盖了约 3倍的该物种基因组。用大麦叶绿体psbA基因和玉米线粒体atp6基因作混合探针 ,检测发现该文库中含细胞器基因组同源序列的克隆数小于 1%。该文库的建成 ,为小麦基因的克隆及基因组学研究提供了技术平台 Using the bacterial artificial chromosome pECBAC1 as a vector, a genomic BAC library of Triticumboeoticum Boiss was constructed. The library contains a total of about 170,000 clones, with an average of 10 4 kb inserts, calculated on the basis of a wild wheat wheat genome of 60 000 Mb, and the library covers about 3-fold the genome of this species. Using barley chloroplast psbA gene and maize mitochondrial atp6 gene as a hybrid probe, the number of cloned organelles homologous sequences in this library was found to be less than 1%. The construction of this library provides a technological platform for the cloning and genomics research of wheat gene