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利用从真养产碱杆菌（Ａｌｃａｌｉｇｅｎｅｓｅｕｔｒｏｐｈｕｓ）Ｈ１６染色体ＤＮＡ中克隆到的催化聚β羟基丁酸酯（ｐｏｌｙβｈｙｄｒｏｘｙｂｕｔｙｒａｔｅ，ＰＨＢ）生物合成的两个关键酶基因：依赖ＮＡＤＰＨ的乙酰乙酰ＣｏＡ还原酶基因（ｐｈｂＢ）以及ＰＨＢ合酶基因（ｐｈｂＣ），同时利用大肠杆菌高效表达载体ｐＫＫ２２３３，构建了嵌合有ｐｈｂＢ和ｐｈｂＣ基因的表达载体ｐＫＣＢ，转入大肠杆菌ＪＭ１０９，通过显微镜观察及气相色谱分析检测ＰＨＢ的合成。在确证克隆基因正确的基础上构建了马铃薯块茎特异性表达载体ｐＰＳＡＧＢ（含ｐｈｂＢ基因）、ｐＢＩＢＧＣ（含ｐｈｂＣ基因）和ｐＰＳＡＧＣＢ（含ｐｈｂＢ和ｐｈｂＣ双基因），转化５个马铃薯品种，经检测获得２０个转基因阳性株系。
Two key enzyme genes for biosynthesis of polyβhydroxybutyrate (PHB), which was cloned from the chromosomal DNA of H16 of Alcaligeneseutrophus, were NADPH-dependent acetoacetyl-CoA (PhbB) and PHB synthase gene (phbC). At the same time, the expression vector pKCB with phbB and phbC genes was constructed by using E. coli high expression vector pKK223-3 and transformed into E. coli JM109. Detection of PHB by Gas Chromatography. The potato tuber-specific expression vector pPSAGB (containing phbB gene), pBIBGC (containing phbC gene) and pPSAGCB (containing phbB and phbC double genes) were constructed based on the correct identification of the cloned genes. Five potato cultivars were transformed into 20 Transgenic positive lines.