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目的:建立中药杜仲中京尼平苷酸(geniposidic acid,GPA)和京尼平苷(geniposide,GP)含量的HPLC测定法。方法:应用HPLC对盐炙杜仲饮片中的GPA和GP进行测定,色谱柱为Agilent TC-C18柱(250 mm×4.6 mm,5μm),柱温为30℃,以甲醇-0.1%磷酸(25∶75,v/v)为流动相,流速为1.0 m L/min,检测波长为240 nm。结果:GPA线性回归方程为YGPA=24.701ρ+39.014(r2=0.999 8),在进样浓度5.12~500μg/m L范闱内呈良好的线性关系;GP线性回归方程为YGP=26.661ρ-25.017(r2=0.999 7),在进样浓度4.506~440μg/m L范围内呈良好的线性关系;GPA和GP加标回收率分别为100.20%和99.80%,其RSD分别为2.15%和2.26%。结论:本法操作简便可行,重复性较好,适用于杜仲药材中京尼平苷酸和京尼平苷的含量测定,及对其药材质量优劣的评价。
OBJECTIVE: To establish an HPLC method for determination of geniposidic acid (GPA) and geniposide (GP) in Eucommia ulmoides. Methods: The GPA and GP of salt-bing Du Zhong decoction were determined by HPLC. The column was Agilent TC-C18 (250 mm × 4.6 mm, 5 μm) with a column temperature of 30 ℃ and methanol-0.1% phosphoric acid 75, v / v) as the mobile phase at a flow rate of 1.0 mL / min with a detection wavelength of 240 nm. Results: The linear regression equation of GPA showed a good linear relationship with the range of 5.12 ~ 500μg / m L in the range of YGPA = 24.701ρ + 39.014 (r2 = 0.999 8). The GP linear regression equation was YGP = 26.661ρ-25.017 (r2 = 0.999 7). The recoveries of GPA and GP were 100.20% and 99.80%, respectively, with RSDs of 2.15% and 2.26%, respectively, in the range of 4.506-440 μg / mL. Conclusion: The method is simple, feasible and reproducible. It is suitable for the determination of geniposidic acid and geniposide in Eucommiae and evaluation of the quality of its medicinal materials.