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为探讨贵州乌蒙烟区不同海拔的烤烟特色形成的分子机理,本文利用实时荧光定量PCR技术对的烤烟碳氮代谢相关酶基因的表达进行检测。结果表明,液泡转化酶(VIN)基因在烤烟移栽后50 d的中海拔表达较强,移栽后60 d高海拔表达比较活跃,烤烟成熟期低海拔表达略强。蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)基因在烤烟旺长期高海拔表达较强,烤烟成熟期低海拔表达较强,中海拔表达较弱。在4个取样时期,海拔越高,蔗糖磷酸化酶(SPP)基因表达越强,高海拔地区烟叶的颗粒结合型淀粉合成酶(GBSSI)基因表达均最强。淀粉分支酶(SBE)基因在烤烟移栽后50、60和70 d高海拔下表达较强,在移栽后80 d低海拔下表达相对较强。从同一时期不同海拔的结果分析,烤烟硝酸还原酶(NR)基因表达强度随海拔降低而逐渐增强。谷氨酰胺合成酶(GS1-3和GS1-5)基因表达在4个取样时期表现为低海拔强于中、高海拔的。
In order to explore the molecular mechanism of the formation of flue-cured tobacco at different altitudes in Guizhou Wumeng tobacco area, the expression of carbon-nitrogen metabolism-related enzymes in flue-cured tobacco was detected by real-time fluorescence quantitative PCR. The results showed that the vacuolar invertase (VIN) gene was highly expressed at mid-seeding 50 days after transplanting of flue-cured tobacco leaves. At 60 days after transplanting, the expression of vacuolar invertase (VIN) gene at altitude was relatively active. Sucrose synthase (SS) and sucrose phosphate synthase (SPS) genes were highly expressed in high and long-term tobacco plants at high altitude. The expression of low altitude and mid-altitude in flue-cured tobacco were stronger at maturity. At 4 sampling periods, the higher the altitude, the stronger the expression of sucrose phosphorylase (SPP) gene and the highest expression of granule-bound starch synthase (GBSSI) gene in tobacco leaves at high altitude. Starch branching enzyme (SBE) gene was expressed at high altitude at 50, 60 and 70 d after transplanting, and was relatively low at 80 d after transplanting. From the results of different altitude at the same period, the expression intensity of nitrate reductase (NR) gene in flue-cured tobacco increased gradually with the altitude decreasing. The gene expression of glutamine synthetase (GS1-3 and GS1-5) showed lower altitude than middle and high altitude in 4 sampling periods.