论文部分内容阅读
【目的】探讨无机磷固、液体培养基及各种基本培养基、磷源等对两株溶磷真菌(FC和H3)溶磷能力的影响,以获得最适合这些菌株发挥溶磷潜力的培养条件。【方法】采用钼蓝比色法测定菌株在无机磷和有机磷液体培养基、简单培养基(SP)、NBRIY、NBRIP和NBRIYP培养基及含不同磷源(磷酸三钙、磷酸铁、磷酸铝、磷酸氢钙和有机磷)、不同浓度磷酸三钙(0.3%、0.5%、0.7%、0.9%和1.1%)培养基的溶磷能力。【结果】FC和H3菌株菌丝在PDA培养基上的最适培养时间为5d。分别在无机磷及有机磷液体培养基、简单培养基(SP)、NBRIY、NBRIP和NBRIYP培养基中培养7d后,FC菌株的溶磷量大小依次为NBRIY>无机磷>NBRIP>SP>有机磷>NBRIYP,H3菌株的溶磷量大小依次为无机磷>NBRIP>SP>NBRIY>有机磷>NBRIYP。以4种不同磷酸盐为磷源,FC菌株对磷酸盐的溶解能力依次为磷酸氢钙>磷酸铝>磷酸铁>磷酸三钙,H3菌株溶解磷酸氢钙的能力较强,其次为磷酸三钙。在含不同浓度磷酸三钙的液体培养基中,两株菌株均能生长并具有溶磷能力,当磷酸三钙浓度为0.5%时,菌株溶磷能力最强。【结论】FC菌株的最适培养基为NBRIY,H3菌株的最适培养基为无机磷培养基。FC和H3菌株对难溶磷酸盐均有溶磷能力,而对磷酸氢钙的溶解能力较强;H3菌株对磷酸三钙的溶解能力较强。
【Objective】 The purpose of this study was to investigate the effects of inorganic phosphate solid and liquid media and various basic media and phosphorus sources on the ability of two phosphate-solubilizing fungi (FC and H3) to dissolve phosphorus, so as to obtain the most suitable culture for these strains condition. 【Method】 Molybdenum blue colorimetric assay was used to determine the bacterial strains in inorganic phosphorus and organophosphorus liquid medium, simple medium (SP), NBRIY, NBRIP and NBRIYP medium and different phosphorus sources (tricalcium phosphate, iron phosphate, aluminum phosphate , Dicalcium phosphate and organophosphorus), different concentrations of tricalcium phosphate (0.3%, 0.5%, 0.7%, 0.9% and 1.1%). 【Result】 The optimal incubation time of mycelium of FC and H3 on PDA medium was 5 days. After being cultured for 7 days in inorganic phosphorus and organophosphorus liquid medium, simple medium (SP), NBRIY, NBRIP and NBRIYP medium, the amount of phosphate solubilizing of FC strains was NBRIY> inorganic phosphorus> NBRIP> SP> > NBRIYP, H3 strains of the amount of dissolved phosphorus in order of inorganic phosphorus> NBRIP> SP> NBRIY> organic phosphorus> NBRIYP. Four different phosphates were used as phosphate source. The ability of FC strains to dissolve phosphates was in the order of dicalcium phosphate> aluminum phosphate> ferric phosphate> tricalcium phosphate, while strain H3 was more capable of dissolving dicalcium phosphate, followed by tricalcium phosphate . In the liquid medium containing different concentrations of tricalcium phosphate, both strains can grow and have the ability to dissolve phosphorus. When the concentration of tricalcium phosphate is 0.5%, the strains have the strongest ability to dissolve phosphorus. 【Conclusion】 The optimal medium for FC strain is NBRIY, and the optimum medium for H3 strain is inorganic phosphorus medium. FC and H3 strains have both ability of phosphate solubilization for phosphate and calcium phosphate, and strain H3 is more soluble for tricalcium phosphate.