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冷冻治疗引起细胞损伤和死亡之机理及主要因素尚未明了,仅有很少文献报道,以形态和功能相结合的酶组织化学方法研究冷冻疗效。本文报告液氮冷冻日本白兔皮肤,于不同时间取材,动态观察冻后组织病理及十三种酶组织化学改变。结果表明:30分钟后,出现典型凝固性坏死,冻后2小时,出现典型酶自溶特征、细胞于冷冻期已死亡。临床上将冷冻分为快速和慢速两型,根据临床标准,本实验属快速冷冻范畴,但从形态及酶活力改变而言,细胞脱水和固缩现象,属于慢速冷冻,因此,临床对此两型分类,值得进一步探讨。脱水是细胞损伤和伤亡之最突出因素,因此,临床宜采用使细胞长期处于超冷状态和反复冻融法。引起细胞严重脱水,致死性损伤,以获最佳疗效。
The mechanism and main factors of cell injury and death induced by cryotherapy are not yet clear. Only few reports have reported the effect of cryotherapy on the basis of enzymatic histochemistry combined with morphology and function. This paper reports the freezing of Japanese white rabbit skin by liquid nitrogen, drawing at different times, dynamically observing frozen histopathology and thirteen kinds of enzyme histochemical changes. The results showed that typical coagulation necrosis occurred 30 minutes later, typical autolytic characteristics appeared 2 hours after freezing, and the cells died during the freezing period. According to the clinical standard, this experiment belongs to the category of rapid freezing. However, in terms of morphological and enzymatic activity changes, cell dehydration and shrinkage phenomenon belong to slow freezing, therefore, clinical The two types of classification, it is worth further study. Dehydration is the most prominent cell injury and casualty factors, therefore, clinical should be used so that the cells in a long-term ultra-cold state and repeated freeze-thaw method. Cause severe cell dehydration, lethal injury, in order to get the best effect.