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目的观察慢性苯中毒患者中毒程度与血清细胞因子变化。方法慢性苯中毒患者53例为中毒组(轻度28例、中度12例、重度13例),对照组为20例健康志愿者。采集每一名研究对象上肢静脉血2 ml,分离血清,采用酶联免疫吸附法(ELISA)检测中毒组与对照组血清中白细胞介素1β(IL-1β)、白细胞介素2(IL-2)、白细胞介素3(IL-3)、白细胞介素6(IL-6)、白细胞介素11(IL-11)及促红细胞生成素(EPO)、血小板生成素(TPO)、肿瘤坏死因子α(TNF-α)含量。结果重度中毒组较对照组及轻、中度组IL-1β、IL-3、IL-6明显降低且IL-2、IL-11增高(P<0.05);TPO表达增高在轻度中毒组高于对照组,但在中、重度中毒组TPO表达降低(P<0.05),重度中毒组较中度中毒组降低明显(P<0.05)。中度中毒组EPO表达增高,但在重度中毒组EPO表达降低(P<0.05);轻、中、重度中毒组TNF-α均高于对照组(P<0.05),重度中毒组表达高于轻、中度中毒组(P<0.05)。结论不同程度苯中毒患者血清细胞因子表达不同,TNF-α、IL-2表达增高与IL-1β、IL-6的降低和中毒严重程度密切相关。表明细胞因子网络失调在慢性苯中毒的发生发展过程中有重要作用。
Objective To observe the degree of poisoning and serum cytokines in patients with chronic benzene poisoning. Methods 53 cases of chronic benzene poisoning poisoning group (28 cases of mild, 12 cases of moderate, severe 13 cases), the control group of 20 healthy volunteers. Serum samples were collected from 2 ml of upper extremity venous blood of each subject and the serum was separated. Serum levels of interleukin-1β (IL-1β) and interleukin-2 (IL-2) in the poisoning and control groups were detected by enzyme-linked immunosorbent assay (ELISA) , Interleukin 3 (IL-3), interleukin 6 (IL-6), interleukin 11 (IL-11) and erythropoietin (EPO), thrombopoietin (TPO), tumor necrosis factor α (TNF-α) content. Results The levels of IL-1β, IL-3 and IL-6 in severe poisoning group were significantly lower than those in control group and light and moderate groups (P <0.05), while the levels of IL-2 and IL- In the control group, TPO expression was lower in the moderate and severe poisoning groups (P <0.05), and severe poisoning group was lower than the moderate poisoning group (P <0.05). The expression of EPO in moderate poisoning group was increased, but the expression of EPO was decreased in severe poisoning group (P <0.05). The levels of TNF-αin light, moderate and severe poisoning group were higher than those in control group (P <0.05) , Moderate poisoning group (P <0.05). Conclusions The levels of serum cytokines are different in patients with different levels of benzene poisoning. The increased expression of TNF-α and IL-2 is closely related to the decrease of IL-1β, IL-6 and the severity of poisoning. Cytokine network disorders that play an important role in the occurrence and development of chronic benzene poisoning.