论文部分内容阅读
本研究以ｒａｓ癌基因表达产物Ｐ２１的单克隆抗体，利用ＡＢＣ免疫组化方法检测胃癌、结肠癌中Ｐ２１的表达情况。结果显示：检测的３８例胃良性病变中，除４例慢性萎缩性胃炎呈阳性反应外，其余病例全部为阴性；３４例胃癌全部为Ｐ２１表达阳性；１８例良性结肠病例中，除２例反应呈弱阳性外，其余为阴性；２８例结肠癌除外一例反应呈阴性反应外，全部为Ｐ２１表达阳性。此结果提示：在胃癌、结肠癌组织中存在着特异性的Ｐ２１表达；ｒａｓｐ２１可作为诊断胃癌、结肠癌的一个较为可靠的标志物、用ＡＢＣ免疫组化方法检测ｒａｓ癌基因产物可能为临床上诊断胃癌、结肠癌提供一种新手段。
In this study, monoclonal antibody against ras oncogene expression product P21 was used to detect the expression of P21 in gastric cancer and colon cancer by ABC immunohistochemistry. The results showed that: Of the 38 cases of benign gastric lesions, except for 4 cases of chronic atrophic gastritis which were positive, all the other cases were negative; 34 cases of gastric cancer were all positive for P21; in 18 cases of benign colon, except 2 cases Weak positive, the rest were negative; 28 cases of colon cancer except a case of a negative reaction, all positive for P21. This result suggests that there is a specific expression of P21 in gastric cancer and colon cancer tissues; rasp21 can be used as a reliable marker for the diagnosis of gastric cancer and colon cancer, and the detection of ras oncogene products by ABC immunohistochemistry may be clinically relevant. Diagnosis of gastric cancer, colon cancer provides a new tool.