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目的探讨双密达莫抑制流感病毒A(H1N1)核蛋白基因(NPG)的作用。方法采用荧光定量RT-PCR法检测药物抑制流感病毒NPG效应。结果药物对MDCK细胞的毒性范围:1∶64稀释为无毒界限。病毒半数细胞感染量(TCID50)为6.5.25μL-1。直线相关性分析结果显示:阈值循环数与不同浓度的定量模板的对数呈负相关(r=-0.999,P<0.05)。病毒对照组RNA水平明显高于实验组[(5.50±1.82)×109拷贝数.μL-1 vs(8.59±2.17)×107拷贝数.μL-1,P<0.01)]。结论双密达莫能阻断流感病毒核蛋白的合成,为防治流感提供了分子实验依据。
Objective To investigate the effect of dipyridamole on the inhibition of influenza virus A (H1N1) nucleoprotein gene (NPG). Methods Fluorescence quantitative RT-PCR method was used to detect the drug inhibit the NPG effect of influenza virus. Results Drug toxicity to MDCK cells Range: 1:64 Diluted to non-toxic limits. The virus half cell infection (TCID50) was 6.5.25 μL-1. The results of linear correlation analysis showed that the number of threshold cycles was negatively correlated with the logarithm of quantitative template with different concentrations (r = -0.999, P <0.05). The level of RNA in the virus control group was significantly higher than that in the experimental group [(5.50 ± 1.82) × 109 copies. ΜL-1 vs (8.59 ± 2.17) × 107 copies. ΜL-1, P <0.01)]. Conclusion Shuangmeidamu can block the synthesis of influenza virus nucleoprotein and provide a molecular experimental basis for the prevention and treatment of influenza.