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该文通过研究豨莶草生品及炮制一至九制品中奇壬醇、豨莶精醇和豨莶苷含量变化规律及指纹图谱对比,为豨莶草炮制研究提供实验基础。采用SunFire-C18色谱柱(4.6 mm×150 mm,5μm),柱温30℃,流动相乙腈(含0.1%甲酸)-水(含0.1%甲酸),流速1 mL·min-1,进样量30μL,检测波长215,320 nm,奇壬醇在2.180~26.16 mg·L-1呈良好的线性关系,平均加样回收率为96.4%,RSD 2.2%;豨莶精醇在2.900~34.80 mg·L-1呈良好的线性关系,平均加样回收率为97.2%,RSD 1.7%;豨莶苷在1.012~6.072 mg·L-1呈良好的线性关系,平均加样回收率为96.3%,RSD 2.4%。该方法准确可靠,重复性好,快速简便;实验结果为豨莶草炮制过程中化学成分变化规律和进一步阐明其疗效的改变奠定了基础。
In this paper, through the study of the change of the content of the odd alcohol, the refined alcohol and the anthocyanin in the products and the comparison of the fingerprints of the genus Quinquefolium from January to September, the experiment provides the experimental basis for the research of Singeing lawn processing. The mobile phase was acetonitrile (containing 0.1% formic acid) -water (containing 0.1% formic acid) and the flow rate was 1 mL · min-1 using a SunFire-C18 column (4.6 mm × 150 mm, 5 μm) 30μL, the detection wavelength was 215,320 nm, and the average linear range was 2.80 ~ 26.16 mg · L-1. The average recoveries were 96.4% and RSD were 2.2%, respectively. The concentrations of elixir ranged from 2.900 to 34.80 mg · L- 1 showed a good linear relationship with an average recovery of 97.2% and a RSD of 1.7%. A good linear relationship was found between 1.012 and 6.072 mg · L -1 of carbendazim. The average recoveries were 96.3% and 2.4% . The method is accurate and reliable with good repeatability and is fast and easy. The experimental results lay the foundation for the change of chemical composition in the process of the process of the process and the elucidation of its curative effect.