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目的:对条叶龙胆根和根茎化学成分进行研究。方法:采用色谱技术针对性地获得酮类化合物,采用ESI-MS和NMR对化合物结构进行鉴定。结果:从该植物中获得9个化合物,分别被鉴定为acremoxanthone D(1),sporormielloside(2),artomandin(3),oliganthaxanthone A(4),oliganthaxanthone B(5),pinetoxanthone(6),polyhongkongenoside A(7),1,5-dihydroxy-2,3,4-trimethoxyxanthone(8)和bannaxanthone I(9)。活性筛选表明化合物1,2,5和9能抑制脂多糖所致巨噬细胞一氧化氮生成,IC_(50)值分别为10.8、5.7、12.5、6.8μmol·L~(-1)。结论:化合物1~9首次从龙胆属植物中分离得到。
Objective: To study the chemical constituents of the roots and rhizomes of Gentianaceae. Methods: Chromosomal compounds were obtained by chromatographic techniques. The structures of compounds were identified by ESI-MS and NMR. Results: Nine compounds were obtained from this plant and identified as acremoxanthone D (1), sporormielloside (2), artomandin (3), oliganthaxanthone A (4), oliganthaxanthone B (5), pinetoxanthone (6), polyhongkongenoside A (7), 1,5-dihydroxy-2,3,4-trimethoxyxanthone (8) and bannaxanthone I (9). Activity screening showed that compounds 1, 2, 5 and 9 could inhibit LPS-induced nitric oxide production in macrophages with IC50 values of 10.8, 5.7, 12.5 and 6.8 μmol·L -1, respectively. Conclusion: Compounds 1 ~ 9 were isolated from Gentiana for the first time.