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目的:本研究采用UPLC建立了同时测定贵州产荭草花药材中没食子酸、儿茶素、高朦胧木素、花旗松素、山柰素-3-O-β-D-葡萄糖苷、槲皮素-3-O-α-L-鼠李糖苷、山柰素-3-O-α-L-鼠李糖苷7种指标成分的含量测定方法。方法:采用ACQU-ITY UPLC BEH C18色谱柱(2.1 mm×150 mm,1.7μm),流动相为0.1%甲酸乙腈-0.1%甲酸水溶液,梯度洗脱,流速为0.3mL.min-1,梯度洗脱,检测波长280 nm,柱温45℃。结果:7个指标成分在选定的范围内线性关系良好(r≥0.999 9),平均加样回收率(n=6)在97.90%～101.4%,RSD 2.3%～3.8%。结论:试验结果显示贵州产荭草花药材所含化学成分基本相同,但被测成分的含量有一定差异,8月中下旬采收的药材中被测成分的含量相对较高,质量较好。
OBJECTIVE: To establish a method for the simultaneous determination of gallic acid, catechin, high hazy lignin, taxifolin, kaempferol-3-O-β-D-glucoside, quercetin -3-O-α-L-rhamnoside, behen-3-O-α-L-rhamnoside seven kinds of indicators of the content of ingredients. Methods: ACQU-ITY UPLC BEH C18 column (2.1 mm × 150 mm, 1.7 μm) was used with the mobile phase consisted of 0.1% formic acid and 0.1% formic acid in water with a gradient of 0.3 mL · min- Off, detection wavelength 280 nm, column temperature 45 ℃. Results: The linearity of the seven index components was good (r≥0.999 9) within the selected range. The average recoveries (n = 6) ranged from 97.90% to 101.4% and RSD from 2.3% to 3.8%. Conclusion: The results showed that the chemical constituents contained in Medicago sativa L. in Guizhou Province were basically the same, but the contents of the tested components were different. The content of the tested components in the middle and late August collected materials was relatively high and the quality was good.