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目的:探讨甲状旁腺素(PTH)基因多态性与中国广东地区部分汉族男性人群骨密度的相关性。方法:随机筛选年龄65～86岁,(70.09±6.69)岁,广州男性264例,采用双能X线吸收法测其全身、腰椎2～4、股骨颈、Ward′三角和大转子区等部位的骨密度(BMD)值,并采用聚合酶链反应限制性片段长度多态性方法检测外周血白细胞基因组PTH基因型。结果:264例受试对象中,PTH基因BB型181例(68.6%)、Bb型73例(27.6%);和bb型10例(3.8%)。PTH基因BstBI基因型分布符合Hardy-Wenbeng平衡定律。三种不同的基因型相互之间的骨密度均无统计学意义(P>0.05)。结论:探讨甲状旁腺素(PTH)基因多态性与广东地区汉族老年男性人群骨密度关系不密切,不能作为筛查和预示骨质疏松症的遗传易感位点。
Objective: To investigate the relationship between PTH gene polymorphism and bone mineral density in some Han Chinese population in Guangdong province, China. Methods: The age ranged from 65 to 86 years old (70.09 ± 6.69) years old and 264 males in Guangzhou were randomly selected. Whole body, lumbar spine 2 ~ 4, femoral neck, Ward ’triangle and the greater trochanter were measured by dual energy X - ray absorptiometry (BMD) were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). PTH genotypes of peripheral blood leukocytes were detected by polymerase chain reaction restriction fragment length polymorphism. RESULTS: Of the 264 subjects, 181 (68.6%) were PTH genotype BB, 73 (27.6%) were Bb, and 10 (3.8%) were bb. PTH BstBI genotype distribution in line with Hardy-Wenbeng equilibrium law. There was no significant difference in BMD between the three genotypes (P> 0.05). Conclusion: To investigate the relationship between parathyroid hormone (PTH) gene polymorphisms and bone mineral density in elderly male Han population in Guangdong Province is not close, and can not be used as a genetic predisposition to screen and predict osteoporosis.