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目的 观察一种新的核苷酸类似物 (E) (2’) 脱氧 氟亚甲基胞苷 (FMdC)在体外的放射增敏作用。方法 在结肠癌 (WiDr)和宫颈癌 (C33 A和C4 I)细胞系进行克隆形成试验。常规照射剂量 2Gy时的放射增敏比 (SERSF2 )定义为 2Gy时对照组存活份数 (SF)和药物处理组SF之比。结果 FMdC增加WiDr细胞的放射敏感性 ,并呈时间依赖关系。照射前用 30nmol/LFMdC处理WiDr细胞 6～ 72h ,在 6、12和 2 4h未能观察到放射增敏作用。放射增敏作用仅在 48和 72h出现 ,其SERSF2 分别为 1.33和 1.79。当高剂量FMdC(30 0nmol/L)处理WiDr细胞 6、12和 2 4h ,各时间点均可见放射增敏效应。相应的放射增敏比SERSF2 分别为 1.19、1.73和 3.5 0。FMdC的放射增敏作用和药物剂量相关 ,2 0～ 5 0nmol/LFMdC处理细胞 48h ,SERSF2 从 1.15增加至 2 .2 8。用 30nmol/LFMdC处理C33 A和C4 I细胞 48h同样可观察到放射增敏作用 ,SERSF2 分别为 1.32和 2 .0 8。结论 FMdC对结肠癌和宫颈癌细胞有明显的放射增敏作用 ,与时间和剂量相关
Objective To observe the radiosensitization of a new nucleotide analog (E) (2 ’) deoxyfluoromethylidenecytidine (FMdC) in vitro. Methods Clonogenicity assays were performed in the cell lines of colon cancer (WiDr) and cervical carcinoma (C33 A and C4 I). The radiosensitization ratio (SERSF2) at 2 Gy of conventional irradiation was defined as the ratio of Survival Number (SF) of control group to SF of drug-treated group at 2Gy. Results FMdC increased the radiosensitivity of WiDr cells in a time-dependent manner. WiDr cells were treated with 30nmol / LFMdC for 6 ~ 72h before irradiation, and radiosensitization was not observed at 6, 12 and 24 hours. Radiosensitization appeared only at 48 and 72h, with SERSF2 of 1.33 and 1.79, respectively. When treated with high dose of FMdC (300 nmol / L) for 6, 12 and 24 h, the radiosensitization effect was observed at all time points. The corresponding radiosensitization ratio SERSF2 was 1.19, 1.73 and 3.5 0, respectively. The radiosensitivity of FMdC was related to the dose of the drug. The cells treated with 20 ~ 50 nmol / L LFdCd for 48h, SERSF2 increased from 1.15 to 2.28. Radiosensitization was also observed with C33 A and C4 I cells treated with 30 nmol / LFMdC for 48 h, with SERSF2 of 1.32 and 2.08, respectively. Conclusion FMdC has obvious radiosensitization effect on colon and cervical cancer cells, which is related to time and dose