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对甘薯品种高系14号及其近缘野生种I.triloba L、和I.lacunosa L,进行原生质体植株再生研究。从离体培养植株的叶柄分离出原生质体,将其培养在含有0.05mg/L 2,4-D和0.5mg/L激动素(KT)的MS培养基中,从原生质体获得了高频率的愈伤组织。培养8-12周后,将直径达2—3mm的小愈伤组织转移到添加0.05mg/L 2,4-D的MS培养基上。转移3-6周后,将愈伤组织进一步转移到添加吲哚乙酸(IAA)和6-苄基嘌呤(BAP)的MS培养基上,一些愈伤组织再生出植株。未再生植株的愈伤组织进一步在MS基本培养基上培养,它们也再生出植株。本研究从I.triloba原生质体获得高频率的植株再生;首次从I.lacunosa原生质体再生出植株;从高系14号原生质体也再生出完整植株。
The regeneration of protoplasts was studied on sweet potato variety GaoXuan 14 and its related wild species I.triloba L and I.lacunosa L. Protoplasts were isolated from petioles of in vitro cultured plants and cultured in MS medium containing 0.05 mg / L 2,4-D and 0.5 mg / L kinetin (KT) to obtain high frequency Callus. After 8-12 weeks of culture, small callus tissues 2-3 mm in diameter were transferred to MS medium supplemented with 0.05 mg / L 2,4-D. After 3-6 weeks of transfer, the callus was further transferred to MS medium supplemented with indole acetic acid (IAA) and 6-benzylpurine (BAP), and some callus regenerated the plants. Callus of non-regenerated plants was further cultured on MS minimal medium, and they also regenerated the plants. In this study, high-frequency plant regeneration was obtained from I.triloba protoplasts; plants were regenerated from I.lacunosa protoplasts for the first time; and whole plants were also regenerated from Protoplast # 14.