【摘 要】
：
The photooxidation damage of lysozyme under 315-375 nm irradiation in the presence of riboflavin was studied by using sodium dodecyl sulfate-polyacrylamide gel
【机 构】
：
Shanghai Institute of Applied Physics,Graduate University of Chinese Academy of Sciences
【基金项目】
：
中国科学院资助项目;国家自然科学基金
论文部分内容阅读
The photooxidation damage of lysozyme under 315-375 nm irradiation in the presence of riboflavin was studied by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Indications showed that the mechanisms and products of oxidative damage were relative to the concentration of riboflavin, the time of irradiation and the ambience. The type Ⅰ process was examined in a nitrogen saturated solution, whereas both type Ⅰ and type Ⅱ were observed in an aerobic atmosphere and type Ⅱ was the dominant process. The study also suggested that antioxidants, such as melatonin, can reduce the damage of lysozyme effectively.
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