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本研究旨在探讨CD64~+中性粒细胞在烧伤并发感染患者体内的变化趋势。在2015年3月至2016年3月期间在广西医科大学附属第一临床医学院烧伤科就诊的患者共100例,男性73例,女性27例;其中观察组(并发感染)50例,对照组(未发生感染)患者50例。对两组患者进行血常规检测、烧伤面积统计,流式细胞仪检测两组患者体内CD64~+中性粒细胞在外周血中的比例,并通过荧光显微镜观察两组患者中性粒细胞CD64表达强度的差异性。我们发现观察组患者外周血CD64~+中性粒细胞比例平均高达(41.5±7.6)%,明显高于对照组的(7.2±5.1)%,差异有统计学意义(p<0.05);荧光显微镜下观察结果显示,观察组患者中性粒细胞表面CD64分子表达强度明显高于对照组。因此我们得出结论,烧伤并发感染患者体内CD64~+中性粒细胞比例明显升高,且表达强度明显高于未感染患者,可以用于日后患者感染的诊断及预测。
The purpose of this study was to investigate the change trend of CD64 + neutrophils in patients with burn-related infection. Between March 2015 and March 2016, there were 100 cases of burn patients attending the First Affiliated Hospital of Guangxi Medical University, including 73 males and 27 females. Among them, 50 cases in the observation group (complicated with infection) and the control group (No infection) in 50 patients. Blood samples were collected from two groups of patients for statistical analysis. The percentage of CD64 ~ + neutrophils in the peripheral blood was measured by flow cytometry. The expression of CD64 in neutrophils was detected by fluorescence microscopy Strength difference. We found that the proportion of CD64 ~ + neutrophils in the peripheral blood of the observation group was (41.5 ± 7.6)%, which was significantly higher than that of the control group (7.2 ± 5.1)%, the difference was statistically significant (p <0.05) Under the observation results showed that the expression of CD64 molecules on the surface of neutrophils in the observation group was significantly higher than that of the control group. Therefore, we conclude that the proportion of CD64 ~ + neutrophils in patients with burn complicated infection is significantly increased, and the expression intensity is significantly higher than that of non-infected patients, which can be used for the diagnosis and prediction of future patient’s infection.