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目的 研究雄性动物性腺的发育和功能与硒的关系。方法 分别以低硒饲料、补硒饲料和常规饲料喂养刚分窝的雄性Ｗ ｉｓｔａｒ 大鼠１４ 周和３９ 周后，分别采用２，３－二氨基萘（ＤＡＮ）荧光分光光度法和化学发光酶免疫法测定血和睾丸硒含量及血浆睾酮水平等指标。结果 饲养１４ 周后，低硒组大鼠不仅血和睾丸硒含量以及睾丸谷胱甘肽过氧化物酶（ＧＰｘ）活性显著低于对照组，而且其精囊腺重量、精囊腺指数和血浆睾酮水平等也显著低于对照组，但其睾丸中脂质过氧化物（ＬＰＯ）水平则显著高于对照组（ Ｐ ＜ ０．０５）。在低硒饲料中补充一定量的硒可在一定程度上纠正这些变化。饲养３９ 周后，低硒组精囊腺指数进一步降低，与对照组的差异达极显著程度（ Ｐ ＜ ０．０１），甚至睾丸指数也降至显著低于对照组。结论 这些结果提示，硒不足对雄性动物性腺的发育和分泌功能有不良影响，适当补硒可作为男性不育症的辅助治疗手段。
Objective To study the relationship between development and function of male gonads and selenium. Methods Male Wistar rats were fed with low-selenium diet, selenium-supplemented diet and conventional diet immediately after 14 weeks and 39 weeks respectively. The fluorescence intensity was calculated by using the fluorescence spectrophotometry of 2,3-diaminonaphthalene (DAN) and the chemiluminescence enzyme Immunoassay to measure blood and testicular selenium content and plasma testosterone levels and other indicators. Results After 14 weeks of rearing, selenium and testicular glutathione peroxidase (GPx) activity in blood serum and testis of rats in low selenium group were significantly lower than those in control group, and seminal vesicle weight, seminal vesicle index and plasma testosterone Were significantly lower than that of the control group, but the level of lipid peroxide (LPO) in testis was significantly higher than that in control group (P <0.05). Supplementation with a certain amount of selenium in low selenium feed can correct these changes to some extent. After 39 weeks of feeding, the selenium index in the selenium-depleted group was further reduced, with a significant difference from the control group (P <0.01), and even the testicular index dropped to a significantly lower level than that of the control group. Conclusions These results suggest that inadequate selenium has an adverse effect on gonadal development and secretion in male animals, and adequate selenium supplementation can be used as an adjunctive treatment for male infertility.