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本文利用电子显微镜及细胞化学技术研究了沙土鼠脑缺血后不同时间海马组织中钙在亚细胞定位分布的变化。实验结果显示:缺血5分钟后最早期的变化为星形细胞突起水肿,以毛细血管周围细胞水肿最明显,细胞内钙分布未见异常变化;缺血30分钟组多数神经细胞固缩,细胞胞浆、胞核及线粒体内有大量钙沉积,钙的蓄积程度与细胞损伤程度一致。实验提示、脑缺血早期的细胞毒性水肿与钙分布的变化无关,但随后由于钙内流,对细胞产生毒性作用而破坏细胞结构的完整性。本文证明,在这类研究中应用草酸——焦锑酸钾电镜细胞化学技术可以显示形态学与机能科学结合起来的优越性。
In this paper, electron microscopy and cytochemical techniques were used to study the distribution of calcium in the hippocampus of gerbils at different time points after cerebral ischemia. The experimental results showed that the earliest changes after 5 minutes of ischemia were astrocytic edema, the most obvious cell edema around the capillaries, no abnormal changes in the intracellular calcium distribution; most of the nerve cells in the 30 minutes of ischemia were shrank and the cells Cytoplasm, nucleus and mitochondria have a large number of calcium deposition, calcium accumulation and cell damage consistent. Experimental results suggest that cytotoxic edema in the early stage of cerebral ischemia has nothing to do with the change of calcium distribution, but later on the calcium influx, toxic effects on the cells and destroy the integrity of the cell structure. This paper demonstrates that the use of oxalic acid-potassium pyroantimonate in these studies demonstrated the superiority of combining morphological and functional science with electron microscopy cytochemistry.