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Metabolism study has been carried out on 1-hydroxy-2,3,5-trimethoxyxanthone(HM-1) and 1-hydroxy-2,3,4,7- tetramethoxyxanthone(HM-2),which are two biologically active ingredients isolated from the Tibetan herb,Halenia elliptica D.Don.,in rat liver microsomes in vitro.A method of high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry(LCMS~n-ESI-IT-TOF) was applied to analyze metabolites of HM-1 and HM-2 on line,and five metabolites were identified containing 1,5-dihydroxy-2,3-dimethoxyxanthone(HM-5),1,7-dihydroxy-2,3,4-trimethoxyxanthone(HM-9),1,4,7- trihydroxy-2,3-dimethoxyxanthone(HM-10),1,4-dihydroxy-2,3,7-trimethoxyxanthone(HM-11) and 1,2-dihydroxy-3,4,7- trimethoxyxanthone(HM-12).Among these metabolites,HM-9,HM-11,and HM-12 were isomers mutually.The results indicated that HM-1 and HM-2 occurred Phase I metabolic reaction of demethylation in rat microsomes in vitro.
Metabolism study has been carried out on 1-hydroxy-2,3,5-trimethoxyxanthone (HM-1) and 1-hydroxy- 2,3,4,7- tetramethoxyxanthone (HM- 2), which are two biologically active ingredients isolated from the Tibetan herb, Halenia elliptica D. Don., in rat liver microsomes in vitro. A method of high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry (LCMS ~ n-ESI-IT-TOF) was applied to analyze metabolites of HM-1 and HM-2 on line, and five metabolites were identified containing 1,5-dihydroxy-2,3-dimethoxyxanthone (HM-5), 1,7-dihydroxy-2,3,4-trimethoxyxanthone (HM-9), 1,4,7-trihydroxy-2,3-dimethoxyxanthone (HM-10), 1,4-dihydroxy-2,3,7-trimethoxyxanthone 3,4,7-trimethoxyxanthone (HM-12) .Among these metabolites, HM-9, HM-11, and HM-12 were isomers. The results indicated that HM-1 and HM- demethylation in rat microsomes in vitro.