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目的:研究精氨酸(Arg)和谷氨酰胺(Gln)对大鼠胃黏膜细胞癌前病变的影响。方法:100只大鼠随机分为对照组、正常组、实验A组、实验B组和实验C组,每组20只。三个实验组和对照组大鼠喂饲N-甲基-N-硝基-亚硝基胍(MNNG),诱导胃黏膜异型增生,建立胃黏膜癌前病变模型。造模成功后,对照组和正常组喂饲能全素,实验A组喂饲能全素添加Arg+Gln组、实验B组喂饲能全素添加Arg、实验C组喂饲能全素添加Gln,实验共8周。观察大鼠的免疫功能、癌基因bcl-2、c-myc的表达和胃黏膜形态学变化。结果:与对照组比,实验A组、B组和C组白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平均下降;CD4+水平和CD4+/CD8+比值均升高;IgA、IgM水平升高,癌基因bcl-2、c-myc表达明显减少,肠上皮化生、异型增生发生率明显下降。实验A组的癌基因bcl-2、c-myc表达、肠上皮化生和异型增生发生率明显低于实验B组和实验C组。与正常组比,对照组IL-6、TNF-α水平显著升高,IgA、IgG、IgM、CD4+水平和CD4+/CD8+比值显著下降,癌基因bcl-2、c-myc表达显著增加,肠上皮化生、异型增生发生率显著升高。结论:Arg和Gln能改善大鼠的免疫功能,通过抑制原癌基因bcl-2、c-myc的表达,减少胃黏膜肠上皮化生和异型增生,阻止胃黏膜癌前病变。
Objective: To study the effect of arginine (Arg) and glutamine (Gln) on gastric mucosal precancerous lesions in rats. Methods: One hundred rats were randomly divided into control group, normal group, experimental group A, experimental group B and experimental group C, with 20 rats in each group. Three groups of experimental rats and control rats were fed with N-methyl-N-nitro-nitrosoguanidine (MNNG) to induce dysplasia of gastric mucosa, and to establish gastric mucosal precancerous lesion model. After successful modeling, the control group and the normal group were fed with vegan. The experimental group A was fed the veg-addition Arg + Gln group, the experimental group B fed the veg-total addition of Arg, and the experimental group C was fed the veg- Gln, the experiment a total of 8 weeks. The immune function, the expression of oncogenes bcl-2, c-myc and gastric mucosal morphology were observed. Results Compared with the control group, the levels of IL-6 and TNF-α in experimental group A, B and C groups were decreased, and the ratio of CD4 + and CD4 + / CD8 + High; IgA, IgM levels, oncogene bcl-2, c-myc significantly reduced the expression of intestinal metaplasia, dysplasia decreased significantly. The incidences of bcl-2, c-myc, intestinal metaplasia and dysplasia in experimental group A were significantly lower than those in experimental group B and C group. Compared with the normal group, the levels of IL-6 and TNF-α in the control group were significantly increased, while the ratios of IgA, IgG, IgM, CD4 + and CD4 + / CD8 + were significantly decreased, while the expression of oncogenes bcl- Metaplasia, dysplasia significantly increased the incidence. CONCLUSION: Arg and Gln can improve the immune function in rats. By inhibiting the expressions of the oncogenes bcl-2 and c-myc, the gastric mucosal intestinal metaplasia and dysplasia can be reduced and the gastric mucosal precancerous lesions can be prevented.