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植物组织培养需用的培养容器过去均采用各种规格的价格昂贵的硬质三角烧瓶或价格较低的广口瓶等。因试验用量大又易损耗,琼脂用量也很大,耗费颇高。经改用塑料盒及液体培养(不加琼脂)方法进行试验,其效果甚佳。现简介如下:一、塑料盒塑料盒选用市场上存放食品的一般透明软质塑料制品(长×宽×高=8×8×5cm)。这种塑料盒不耐高温高压(100℃水倒入立即变形不能再使用)故采用三种消毒剂:(1)70％酒精,(2)3％洗消剂;(3)1:50新洁尔灭。三者分别消毒1小时、3小时、24小时。之后取出放在超净台上吹干,注入冷却至
Culture vessels used in plant tissue culture have historically been using a variety of expensive hard conical flasks or lower cost jars and the like. Because of the large amount of test and easy to consume, the amount of agar is also very large, high cost. After the switch to plastic boxes and liquid culture (without agar) method for testing, the effect is very good. The brief introduction is as follows: First, the plastic box plastic box used in the market to store food generally transparent soft plastic products (L × W × H = 8 × 8 × 5cm). This plastic box is not resistant to high temperature and pressure (100 ℃ water into the immediate deformation can not be used) Therefore, the use of three kinds of disinfectants: (1) 70% alcohol, (2) 3% decontamination; . Three were disinfected 1 hour, 3 hours, 24 hours. After removal on the clean bench blow dry, injection cooled to